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Anti-NFKBID Polyclonal Antibody
Overview
Catalog No. HV187014
Host species Rabbit
Species reactivity Human, Mouse
Immunogen E. coli - derived recombinant Human NFKBID (Asp46-Val285).
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Clonality Polyclonal
Isotype IgG
Applications ELISA, IHC, WB
Target NFKB inhibitor delta, IkB-delta, TA-NFKBH, NF-kappa-B inhibitor delta, IkappaBNS, I-kappa-B-delta, T-cell activation NFKB-like protein, IKBNS, IkappaBdelta, NFKBID
Purification Purified by antigen affinity column.
Accession Q8NI38
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Product Usage Information
ApplicationDilution
ELISA1:5000-1:20000
IHC1:50-1:500
WB1:500-1:2000
Note For research use only.
Images


Immunofluorescence
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting NFKBID (HV187014) at a concentration of 12.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of NFKBID was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).