| Catalog No. | HV095014 | ||||||||
| Host species | Rabbit | ||||||||
| Species reactivity | Human, Mouse, Rat, Bos taurus, Macaca fascicularis, Pongo abelii | ||||||||
| Immunogen | E. coli - derived recombinant Human CACYBP (Ala2-Phe228). | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. | ||||||||
| Clonality | Polyclonal | ||||||||
| Isotype | IgG | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Target | S100A6-binding protein, SIP, Siah-interacting protein, CacyBP, CACYBP, hCacyBP, S100A6BP, Calcyclin-binding protein | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Accession | Q9HB71 | ||||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Product Usage Information |
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| Note | For research use only. |
Anti-CACYBP Polyclonal Antibody
Overview
Images
 Immunofluorescence |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CACYBP (HV095014) at a concentration of 14.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CACYBP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |
 Western blot |
Western blot analysis was performed using anti-CACVBP polyclonal antibody at 1μg/ml on various samples. Lane 1: K562 cell lysate Lane 2: HCT116 cell lysate Lane 3: MCF7 cell lysate Lane 4: 293T cell lysate Lane 5: HepG2 cell lysate Lane 6: HL60 cell lysate Lane 7: Jurkat cell lysate Lane 8: Rat brain lysate Lane 9: Rat liver lysate |