| Catalog No. | HB104014 | ||||||||
| Host species | Rabbit | ||||||||
| Species reactivity | Human, Macaca mulatta | ||||||||
| Immunogen | E. coli - derived recombinant Human CD326/EPCAM (Gln24-Lys265). | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. | ||||||||
| Clonality | Polyclonal | ||||||||
| Isotype | IgG | ||||||||
| Applications | ELISA, IHC, WB, IF | ||||||||
| Target | Epithelial glycoprotein 314, hEGP314, Major gastrointestinal tumor-associated protein GA733-2, EGP314, TACSTD1, Adenocarcinoma-associated antigen, TROP1, EPCAM, Epithelial glycoprotein, KS 1/4 antigen, Tumor-associated calcium signal transducer 1, M4S1, CD326, Ep-CAM, EGP, Epithelial cell adhesion molecule, M1S2, KSA, Cell surface glycoprotein Trop-1, GA733-2, Epithelial cell surface antigen, MIC18 | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||||
| Accession | P16422 | ||||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Product Usage Information |
|
||||||||
| Note | For research use only. |
Anti-CD326/EPCAM Polyclonal Antibody
Overview
Images
 Immunofluorescence |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting EpCAM (HB104014) at a concentration of 20.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of EpCAM was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |
 Western blot |
Western blot analysis was performed using anti-EPCAM polyclonal antibody at 1μg/ml on various samples. Lane 1: HCT116 cell lysate Lane 2: MCF7 cell lysate Lane 3: HT-29 cell lysate Lane 4: Mouse colon lysate |