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Anti-Human EGFR Reference Antibody (Zatuximab, RUO)
Overview
Catalog No. HF004106
Host species Chimeric
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact the lab for this information.
Expression system Mammalian Cells
Accession P00533
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name Futuximab, DS992, Sym004 (combination of modotuximab and futuximab), 1310460-86-6
Note For research use only. Not suitable for clinical or therapeutic use.
Images


SEC-HPLC		 SEC-HPLC detection for Research Grade Zatuximab.

Bioactivity		 Detects EGFR/ERBB1/HER1 in indirect ELISAs.

SDS-PAGE		 SDS-PAGE for Research Grade Zatuximab

Flow CytoMetry		 Flow-cytometry using APC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human EGFR monoclonal antibody (Catalog: HF004106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using PE anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR monoclonal antibody (Catalog: HF004106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: HF004106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.