Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Western blot analysis was performed using anti-MAP2K4 polyclonal antibody at 1μg/ml on various samples. Lane 1: RAW264.7 cell lysate Lane 2: NIH3T3 cell lysate Lane 3: 293T cell lysate Lane 4: HepG2 cell lysate Lane 5: Jurkat cell lysate Lane 6: K562 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain lysate