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Anti-TWSG1 Polyclonal Antibody
Overview
Catalog No. HV536014
Host species Rabbit
Species reactivity Human, Mouse, Rat
Immunogen E. coli - derived recombinant Human TWSG1 (Cys26-Phe223).
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Clonality Polyclonal
Isotype IgG
Applications ELISA, IHC, WB
Target Twisted gastrulation protein homolog 1, TSG, TWSG1
Purification Purified by antigen affinity column.
Accession Q9GZX9
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Product Usage Information
Application Dilution
ELISA1:5000-1:20000
IHC1:50-1:500
WB1:500-1:2000
Note For research use only.
Images


Immunofluorescence
A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting TWSG1 (HV536014) at a concentration of 21 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of TWSG1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Western blot
Western blot analysis was performed using anti-TWSG1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate