| Catalog No. | HV728014 | ||||||||
| Host species | Rabbit | ||||||||
| Species reactivity | Human, Mouse, Rat | ||||||||
| Immunogen | E. coli - derived recombinant Human RTCB (Met1-Gly505). | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. | ||||||||
| Clonality | Polyclonal | ||||||||
| Isotype | IgG | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Target | C22orf28, RNA-splicing ligase RtcB homolog, RTCB, 3'-phosphate/5'-hydroxy nucleic acid ligase | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Accession | Q9Y3I0 | ||||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Product Usage Information |
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| Note | For research use only. |
Anti-RTCB Polyclonal Antibody
Overview
Images
 Immunofluorescence |
A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting RTCB (HV728014) at a concentration of 31.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of RTCB was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |
 Western blot |
Western blot analysis was performed using anti-RTCB polyclonal antibody at 1μg/ml on various samples. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: Mouse liver lysate |