Anti-ALOX12B Polyclonal Antibody

Overview

Catalog No.

HF742014

Host species

Rabbit

Species reactivity

Human, Mouse, Rat

Immunogen

E. coli - derived recombinant Human ALOX12B (Leu479-Ile701).

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Target

ALOX12B, Epidermis-type lipoxygenase 12, Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase

Purification

Purified by antigen affinity column.

Accession

O75342

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Images

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALOX12B (HF742014) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALOX12B was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).