Anti-DHPS Polyclonal Antibody
Overview
| Catalog No. |
HW482014 |
| Host species |
Rabbit |
| Species reactivity |
Human, Mouse, Rat |
| Immunogen |
E. coli - derived recombinant Human DHPS (Met1-Asp369). |
| Form |
Liquid |
| Storage buffer |
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| Clonality |
Polyclonal |
| Isotype |
IgG |
| Applications |
ELISA, IHC, WB |
| Target |
DHPS, Deoxyhypusine synthase, DHS, DS |
| Purification |
Purified by antigen affinity column. |
| Accession |
P49366 |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Product Usage Information |
| Application |
Dilution |
| ELISA | 1:5000-1:20000 | | IHC | 1:50-1:500 | | WB | 1:500-1:2000 |
|
| Note |
For research use only. |
Images
 Immunofluorescence |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting DHPS (HW482014) at a concentration of 24 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of DHPS was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |