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Anti-Human DSC2 Polyclonal Antibody
Overview
Catalog No.
HX236014
Host species
Rabbit
Species reactivity
Human
Immunogen
E. coli - derived recombinant Human DSC2 (Arg136-Cys471).
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, IHC, WB
Target
Desmosomal glycoprotein II, Desmocollin-2, DSC2, DSC3, Cadherin family member 2, CDHF2, Desmosomal glycoprotein III, Desmocollin-3
Purification
Purified by antigen affinity column.
Accession
Q02487
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Images

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting DSC2 (HX236014) at a concentration of 93.35 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of DSC2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).