Anti-AXIN1 Polyclonal Antibody

Overview

Catalog No.

HT232014

Host species

Rabbit

Species reactivity

Human, Mouse, Rat

Immunogen

E. coli - derived recombinant Human AXIN1 (Ser653-Asp862).

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Target

AXIN, Axin-1, hAxin, Axis inhibition protein 1, AXIN1

Purification

Purified by antigen affinity column.

Accession

O15169

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Images

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting AXIN1 (HT232014) at a concentration of 13.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of AXIN1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).