Anti-CSPG4 Polyclonal Antibody

Overview

Catalog No.

HV349014

Host species

Rabbit

Species reactivity

Human, Mouse, Rat

Immunogen

E. coli - derived recombinant Human CSPG4 (Ser1705-Ile1942).

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Target

Chondroitin sulfate proteoglycan 4, CSPG4, Chondroitin sulfate proteoglycan NG2, MCSP, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan

Purification

Purified by antigen affinity column.

Accession

Q6UVK1

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Images

Immunofluorescence

U2OS cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CSPG4 (HV349014) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CSPG4 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).