Anti-HSPA5 Polyclonal Antibody
Overview
| Catalog No. |
HY489024 |
| Host species |
Rabbit |
| Species reactivity |
Human, Mouse |
| Immunogen |
E. coli - derived recombinant Human HSPA5 (Leu351-Leu654). |
| Form |
Liquid |
| Storage buffer |
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| Clonality |
Polyclonal |
| Isotype |
IgG |
| Applications |
ELISA, IHC, WB |
| Target |
Heat shock protein 70 family protein 5, 78 kDa glucose-regulated protein, HSPA5, Binding-immunoglobulin protein, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Endoplasmic reticulum chaperone BiP, GRP78, BiP, Immunoglobulin heavy chain-binding protein |
| Purification |
Purified by antigen affinity column. |
| Accession |
P11021 |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Product Usage Information |
| Application |
Dilution |
| ELISA | 1:5000-1:20000 | | IHC | 1:50-1:500 | | WB | 1:500-1:2000 |
|
| Note |
For research use only. |
Images
 Western blot |
Western blot analysis was performed using anti-HSPA5 polyclonal antibody at 1ug/mL on various samples. Lane 1: HepG2 cell lysate Lane 2: NIH3T3 cell lysate Lane 3: 4T1 cell lysate Lane 4: MCF-7 cell lysate Lane 5: Jurkat cell lysate Lane 6: PC-12 cell lysate Lane 7: Hela cell lysate Lane 8: A549 cell lysate Lane 9: Mouse brain lysate Lane 10: Mouse liver lysate Lane11: Rat brain lysate Lane12: Rat liver lysate
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