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Research Grade Panitumumab
Overview
Catalog No. HF004036
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact the lab for this information.
Expression system Mammalian Cells
Accession P00533
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name ABX-EGF, E7.6.3, 339177-26-3
Note For research use only. Not suitable for clinical or therapeutic use.
Description

Panitumumab (HF004036) is a research-grade recombinant antibody targeting HER1. Produced in mammalian cells with native-like glycosylation.
Highlights
  • ●Research Grade — For PK/PD studies, assay development, and ADA research.
  • ●Native Glycosylation — Mammalian expression ensures native-like patterns.

Images


SEC-HPLC		 SEC-HPLC detection for Research Grade Panitumumab.

Bioactivity		 Detects EGFR/ERBB1/HER1 in indirect ELISAs.

Flow Cytometry		 Flow-cytometry using anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR monoclonal antibody (Catalog HF004036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry		 Flow-cytometry using anti-human EGFR antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR monoclonal antibody (Catalog HF004036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for Research Grade Panitumumab.

Flow CytoMetry		 Flow-cytometry using anti-human EGFR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFR monoclonal antibody (Catalog: HF004036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.