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Research Grade Certolizumab
Overview
Catalog No. HF879016
Host species Humanized
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype Fab'-IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
Purification Protein L purified from cell culture supernatant.
Endotoxin level Please contact the lab for this information.
Expression system Mammalian Cells
Accession P01375
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name CDP870, PHA-738144, Certolizumabpegol, 428863-50-7
Note For research use only. Not suitable for clinical or therapeutic use.
Description

Certolizumab (HF879016) is a research-grade recombinant antibody targeting CD2. Produced in mammalian cells with native-like glycosylation.
Highlights
  • ●Research Grade — For PK/PD studies, assay development, and ADA research.
  • ●Native Glycosylation — Mammalian expression ensures native-like patterns.

Images


Bioactivity		 Detects Human TNFa/TNF-alpha in indirect ELISAs.

SDS-PAGE		 SDS-PAGE for Research Grade Certolizumab

Flow Cytometry		 Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry using APC anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry using PE anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry using FITC anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.