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Research Grade Enfortumab
Overview
Catalog No. HV985016
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact the lab for this information.
Expression system Mammalian Cells
Accession Q96NY8
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative nameAGS-22CE, AGS-22M, AGS-22M6E, unconjugated:AGS-22C3orAGSM6, 1448664-46-7
Note For research use only. Not suitable for clinical or therapeutic use.
Images


SEC-HPLC
SEC-HPLC detection for Research Grade Enfortumab.

Bioactivity
Detects NECTIN4/PVRL4 in indirect ELISAs.

SDS-PAGE
SDS-PAGE for Research Grade Enfortumab.

Flow CytoMetry
Flow-cytometry using anti-human Nectin 4 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human Nectin 4 monoclonal antibody (Catalog: HV985016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot
Western blot analysis was performed using anti-NECTIN4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human NECTIN4 (Catalog No: HV985011)
Lane 2: negative control