Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins
PNGase F
Overview
Catalog No.
JN842012
Form
Liquid
Storage buffer
20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol
Purity
>95% as determined by SDS-PAGE.
Applications
Removal of high mannose N-glycans from glycoproteins
Endotoxin level
Please contact with the lab for this information.
Expression system
E. coli
Protein length
PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
Nature
Recombinant
Predicted molecular weight
37.08 kDa
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Alternative name
83534-39-8
Species
Elizabethkingia miricola
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Note
For research use only.
Description
Images
SDS-PAGE
SDS-PAGE for PNGase F
Bioactivity
The deglycosylation of protein detect by SDS-PAGE