| Catalog No. | BX112011 |
| Form | Liquid |
| Storage buffer | 50 mM Tris-HCl, 50%Glycerol, pH 8.0 |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | Fusion Protein Cleavage, Protein Purification, Protein Digestion |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | Yeast |
| Protein length | Enterokinase is cloned from bovine and expressed in Yeast. |
| Nature | Recombinant |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Species | Bovine |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Predicted molecular weight | 22.11 kDa |
| Note | For research use only. |
| Biological activity | One unit (1 U) of enzyme activity is defined as the amount of enzyme that cleaves 95% of 50 µg of a fusion protein containing the recognition site within 16 hours at 25 °C in 50 mM Tris-HCl (pH 8.0). |
Enterokinase
Overview
Description
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
Images
 SDS-PAGE |
SDS-PAGE for Enterokinase |