Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0142)

Catalog No.

MV974013

Host species

Mouse

Species reactivity

Mouse

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG2a, lambda

Applications

Blocking, ELISA, FCM

Target

B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

Please contact with the lab for this information.

Accession

Q9EP73

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0142

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only.

SDS-PAGE	SDS-PAGE for Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0142).
Flow CytoMetry	Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD274 monoclonal antibody (Catalog: MV974013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: MV974013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD274 monoclonal antibody (Catalog: MV974013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.