3C Protease_Datasheet

Catalog No.	VK794012
Form	Liquid
Storage buffer	50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
Purity	>95% as determined by SDS-PAGE.
Applications	Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Endotoxin level	Please contact with the lab for this information.
Expression system	E. coli
Protein length	3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
Nature	Recombinant
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Species	HRV-14
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Predicted molecular weight	47.46 kDa
Note	For research use only.

3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Activity definition: 1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 μg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C.

SDS-PAGE

SDS-PAGE for 3C Protease

Bioactivity

M: Marker
1: Control
2: Fusion protein digest with 3C protease

Case study		Formula
concentration	1 mg/ml	c mg/ml
volume	100 µl	V ml
substrate	100 µg	cV mg
10X Buffer	11 µl	V/9 ml
3C Protease	1 µl	cV/100 ml

Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.