3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage. <br> Activity definition: 1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 μg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C.
3C Protease
Overview
Catalog No.
VK794012
Form
Liquid
Storage buffer
50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
Purity
>95% as determined by SDS-PAGE.
Applications
Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Endotoxin level
Please contact with the lab for this information.
Expression system
E. coli
Protein length
3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
Nature
Recombinant
Predicted molecular weight
47.46 kDa
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Species
HRV-14
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Note
For research use only.
Description
Images
SDS-PAGE
SDS-PAGE for 3C Protease
Bioactivity
M: Marker
1: Control
2: Fusion protein digest with 3C protease
1: Control
2: Fusion protein digest with 3C protease
| Case study | Formula | |
|---|---|---|
| concentration | 1 mg/ml | c mg/ml |
| volume | 100 µl | V ml |
| substrate | 100 µg | cV mg |
| 10X Buffer | 11 µl | V/9 ml |
| 3C Protease | 1 µl | cV/100 ml |
Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.