| Catalog No. | HW299013 | ||||||
| Host species | Mouse | ||||||
| Species reactivity | Homo sapiens (Human) | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS, pH 7.4. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | IgG1, kappa | ||||||
| Applications | Blocking, ELISA, ICC, IF, FCM, WB | ||||||
| Target | MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | P50281 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Clone ID | LEM-2/15 | ||||||
| Product Usage Information |
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| Note | For research use only. |
Anti-Human MMP14 Antibody (LEM-2/15)
Overview
Images
 Bioactivity |
Detects MMP14 in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Anti-Human MMP14 Antibody (LEM-2/15). |
 Flow Cytometry |
Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 antibody monoclonal antibody (Catalog # HW299013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human MMP14 (Catalog No: HW299022) Lane 2: negative control |