Anti-Human ADAMTS13 Antibody (3H9)
Overview
Catalog No.
HV675013
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, lambda
Applications
Blocking, ELISA, FCM, SPR
Target
A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, 3.4.24.87, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease, ADAMTS13, C9orf8, UNQ6102/PRO20085
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
Q76LX8
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
3H9
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Anti-Human ADAMTS13 Antibody (3H9).
Flow Cytometry
Flow-cytometry using anti-human ADAMTS13 antibody. ADAMTS13 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog # HV675013, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human ADAMTS13 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ADAMTS13 antibody monoclonal antibody (Catalog # HV675013, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC- conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using PE anti-human ADAMTS13 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAMTS13 monoclonal antibody (Catalog: HV675013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.