| Catalog No. | HF827010 |
| Host species | Human |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | ELISA, FCM, Neutralization |
| Target | Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2, IFN-Alpha, IFNA2A, IFNA2B, IFNA2C, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFNA1, IFNA13, IFN-α4, IFN-α5, IFN-α6, IFN-α7, IFN-α8, IFN-αl0, IFN-α14, IFN-α16, IFN-α17, IFN-α21 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P01563 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | Iv0001 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001)
Overview
Images
 Bioactivity |
Detects Human IFN α2/IFNA2 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human IFN α2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human IFN α2 antibody.SHSY-5Y cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001). |
 Western blot |
Western blot analysis was performed using anti-IFNA2/IFN-alpha-2 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 50ng Lane 2: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 30ng Lane 3: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 20ng Lane 4: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 10ng |