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InVivoMAb Anti-Human IL2 (Iv0020)
Overview
Catalog No. HX011010
Host species Mouse
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1
Applications ELISA, FCM, Neutralization
Target IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P60568
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDIv0020
Note For research use only. Not suitable for clinical or therapeutic use.
Images


Bioactivity
Detects IL2 in indirect ELISAs.

Flow Cytometry
Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # HX011010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human IL2 (Iv0020).

Western blot
Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL2 (Catalog No: HX011012)
Lane 2: negative control