| Catalog No. | HY328010 |
| Host species | Human |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | ELISA, FCM, Neutralization |
| Target | Hybridoma growth factor, BSF-2, IFN-beta-2, CDF, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, Interferon beta-2, IFNB2, CTL differentiation factor |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P05231 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | Iv0022 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| RRID | InVivoMAb Anti-Human IL6 (Iv0022) (abinScience Cat# HY328010, RRID:AB_3725914) |
InVivoMAb Anti-Human IL6 (Iv0022)
Overview
Description
InVivoMAb Anti-Human IL6 (Iv0022) [Iv0022] (HY328010) is a human monoclonal antibody detecting Hybridoma growth factor in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
- ●Multi-Application — Validated across multiple applications.
- ●Flow Cytometry — Validated for flow cytometry applications.
Images
 Bioactivity |
SEC-HPLC detection for InVivoMAb Anti-Human IL6 (ABV0244). |
 Flow Cytometry |
Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for InVivoMAb Anti-Human IL6 (Iv0022). |
 Western blot |
Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng |