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InVivoMAb Anti-Human CSF1/M-CSF (Iv0052)
Overview
Catalog No. HY515010
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications ELISA, FCM, Neutralization
Target CSF1, Macrophage colony-stimulating factor 1, CSF-1, Lanimostim, M-CSF, MCSF
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P09603
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID Iv0052
Note For research use only. Not suitable for clinical or therapeutic use.
Description

InVivoMAb Anti-Human CSF1/M-CSF (Iv0052) [Iv0052] (HY515010) is a human monoclonal antibody detecting CSF1 in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●Flow Cytometry — Validated for flow cytometry applications.

Images


Bioactivity		 SEC-HPLC detection for InVivoMAb Anti-Human CSF1/M-CSF (ABV0114).

Flow Cytometry		 Flow-cytometry using anti-human CSF1 antibody.MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF1 antibody monoclonal antibody (Catalog # HY515010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for InVivoMAb Anti-Human CSF1/M-CSF (Iv0052).

Flow CytoMetry		 Flow-cytometry using FITC anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using PE anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using APC anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.