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InVivoMAb Anti-Human CSF2/GM-CSF (Iv0053)
Overview
Catalog No. HY213010
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications ELISA, FCM, Neutralization
Target Granulocyte-macrophage colony-stimulating factor, Sargramostim, GMCSF, CSF, Colony-stimulating factor, CSF2, GM-CSF, Molgramostin
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P04141
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID Iv0053
Note For research use only. Not suitable for clinical or therapeutic use.
Description

InVivoMAb Anti-Human CSF2/GM-CSF (Iv0053) [Iv0053] (HY213010) is a human monoclonal antibody detecting Granulocyte-macrophage colony-stimulating factor in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●Flow Cytometry — Validated for flow cytometry applications.

Images


Bioactivity		 SEC-HPLC detection for InVivoMAb Anti-Human CSF2/GM-CSF (ABV0027).

Flow Cytometry		 Flow-cytometry using anti-human CSF2 antibody.CSF2 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry		 Flow-cytometry using anti-human CSF2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for InVivoMAb Anti-Human CSF2/GM-CSF (Iv0053).

Flow CytoMetry		 Flow-cytometry using FITC anti-human CSF2 antibody. MDA-MB-231 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CSF2 monoclonal antibody (Catalog: HY213010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.