InVivoMAb Anti-Human CSF2/GM-CSF (Iv0053)

Catalog No.	HY213010
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1, kappa
Applications	ELISA, FCM, Neutralization
Target	Granulocyte-macrophage colony-stimulating factor, Sargramostim, GMCSF, CSF, Colony-stimulating factor, CSF2, GM-CSF, Molgramostin
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	P04141
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	Iv0053
Note	For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	SEC-HPLC detection for InVivoMAb Anti-Human CSF2/GM-CSF (ABV0027).
Flow Cytometry	Flow-cytometry using anti-human CSF2 antibody.CSF2 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human CSF2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for InVivoMAb Anti-Human CSF2/GM-CSF (Iv0053).
Flow CytoMetry	Flow-cytometry using FITC anti-human CSF2 antibody. MDA-MB-231 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CSF2 monoclonal antibody (Catalog: HY213010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.