| Catalog No. | HF909010 |
| Host species | Human |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG2, kappa |
| Applications | ELISA, FCM, Neutralization |
| Target | Beta-nerve growth factor, NGFB, Beta-NGF, NGF |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P01138 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | Iv0066 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066)
Overview
Images
 Bioactivity |
SEC-HPLC detection for InVivoMAb Anti-Human NGF/Beta-NGF (ABV0233). |
 Flow Cytometry |
Flow-cytometry using anti-human NGF antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NGF antibody monoclonal antibody (Catalog # HF909010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066). |
 Western blot |
Western blot analysis was performed using anti-NGF monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human NGF (Catalog No: HF909011) Lane 2: negative control |