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InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066)
Overview
Catalog No. HF909010
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2, kappa
Applications ELISA, FCM, Neutralization
Target Beta-nerve growth factor, NGFB, Beta-NGF, NGF
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P01138
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID Iv0066
Note For research use only. Not suitable for clinical or therapeutic use.
RRID InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066) (abinScience Cat# HF909010, RRID:AB_3725967)
Description

InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066) [Iv0066] (HF909010) is a human monoclonal antibody detecting Beta-nerve growth factor in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●Flow Cytometry — Validated for flow cytometry applications.

Images


Bioactivity		 SEC-HPLC detection for InVivoMAb Anti-Human NGF/Beta-NGF (ABV0233).

Flow Cytometry		 Flow-cytometry using anti-human NGF antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NGF antibody monoclonal antibody (Catalog # HF909010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066).

Western blot		 Western blot analysis was performed using anti-NGF monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human NGF (Catalog No: HF909011)
Lane 2: negative control