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InVivoMAb Anti-Human CD228/MELTF (Iv0104)
Overview
Catalog No.
HY094010
Host species
Human
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG1
Applications
ELISA, FCM, Neutralization
Target
CD228, Melanotransferrin, Melanoma-associated antigen p97, MAP97, MFI2, MELTF
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P08582
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
Iv0104
Note
For research use only. Not suitable for clinical or therapeutic use.
Images

Bioactivity

Detects CD228/MELTF in indirect ELISAs.

Flow Cytometry

Flow-cytometry using anti-human CD228 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CD228/MELTF (Iv0104).

Flow CytoMetry

Flow-cytometry using PE anti-human CD228 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD228 monoclonal antibody (Catalog: HY094010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry

Flow-cytometry using FITC anti-human CD228 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD228 monoclonal antibody (Catalog: HY094010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry

Flow-cytometry using APC anti-human CD228 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD228 monoclonal antibody (Catalog: HY094010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.