InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120)
Overview
Catalog No.
HY339010
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
ELISA, FCM, Neutralization
Target
Liver-type arginase, ARG1, Type I arginase, Arginase-1
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P05089
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
Iv0120
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
Bioactivity
Detects Human ARG1/Arginase-1 in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human Arginase 1 antibody.M2 macrophages derived from THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # HY339010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human Arginase 1 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # HY339010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120).
Flow CytoMetry
Flow-cytometry using PE anti-human Arginase 1 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human Arginase 1 monoclonal antibody (Catalog: HY339010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.