Research Grade Ivonescimab

Каталожный номер	HB941056
Хозяинский вид	Humanized
Реактивность видов	Human
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4.
Чистота	>95% purity as determined by SDS-PAGE.
Клоональность	Monoclonal
Изотип	IgG1-kappa-[scFv]2
Применения	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Цель	Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A, Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1, CD279
Очистка	Protein A/G purified from cell culture supernatant.
Уровень эндотоксинов	Please contact the lab for this information.
Система экспрессии	Mammalian Cells
Номер доступа	P15692 & Q15116
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name	TetravalentBispecific, AK112, AK-112, AK1122428381-53-5
Примечание	For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	Detects VEGF165 in indirect ELISA.
Bioactivity	Detects recombinant human PD1 (Cat No.: HS870021) in indirect ELISA.
Flow CytoMetry	Flow-cytometry using FITC anti-human VEGFA&PD1 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA&PD1 monoclonal antibody (Catalog: HB941056, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011) Lane 2: negative control
Flow cytometry	Flow-cytometry using anti-human VEGFA antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA monoclonal antibody (Catalog HB941056, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.