InVivoMAb Anti-Human STAT3 Antibody (Iv0126)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Applications
ELISA, FCM, Neutralization
Target
Signal transducer and activator of transcription 3, APRF, STAT3, Acute-phase response factor
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
Flow Cytometry
Flow-cytometry using anti-human STAT3 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human STAT3 antibody monoclonal antibody (Catalog # HW708010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human STAT3 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human STAT3 antibody monoclonal antibody (Catalog # HW708010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human STAT3 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human STAT3 antibody monoclonal antibody (Catalog # HW708010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human STAT3 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human STAT3 antibody monoclonal antibody (Catalog # HW708010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.