| Catalog No. | HC491013 | ||||||
| Host species | Mouse | ||||||
| Species reactivity | Human, Mouse | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS, pH 7.4. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | IgG1, kappa | ||||||
| Applications | ELISA, FCM, WB | ||||||
| Target | BAX, Bcl2-L-4, BCL2L4, Bcl-2-like protein 4, Apoptosis regulator BAX | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | Q07812 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Clone ID | 6A7 | ||||||
| Product Usage Information |
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| Note | For research use only. |
Anti-Human BAX Antibody (6A7)
Overview
Images
 Bioactivity |
SEC-HPLC detection for Anti-Human BAX Antibody (6A7). |
 Flow Cytometry |
Flow-cytometry using anti-human BAX antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human BAX antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human BAX antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human BAX antibody monoclonal antibody (Catalog # HC491013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human BAX Antibody (6A7). |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human BAX antibody. TNFa stimulated U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human BAX monoclonal antibody (Catalog: HC491013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |