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Research Grade Reozalimab
概要
カタログ番号 HV974216
宿主種 Human
種反応性 Human
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4.
純度 >95% purity as determined by SDS-PAGE.
クローン性 Monoclonal
アイソタイプ Ig(G1-kappa_G1-lambda2)
アプリケーション ELISA, Bioactivity: FACS, Functional assay, Research in vivo
ターゲット B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274, Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1, CD279
精製 Protein A/G purified from cell culture supernatant.
エンドトキシンレベル Please contact the lab for this information.
発現システム Mammalian Cells
アクセッション番号 Q9NZQ7 & Q15116
安定性と保存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name Bispecific, IBI318, IBI-318, IBI318, 2445259-99-2
注意事項 For research use only. Not suitable for clinical or therapeutic use.
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Bioactivity
Detects PDL1 in indirect ELISA.

Bioactivity
Detects recombinant human PD1 (Cat No.: HS870021) in indirect ELISA.

Flow CytoMetry
Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: HV974216) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot
Western blot analysis was performed using anti-CD274/PD-L1/B7-H1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD274/PD-L1/B7-H1 (Catalog No: HV974011)
Lane 2: negative control