InVivoMAb Anti-Mouse CD274/PD-L1/B7-H1 Antibody (10F.9G2)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Applications
in vivo PD-L1 blockade, in vitro PD-L1 blockade, IF, IHC-F, FCM, WB
Target
Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
Bioactivity
Detects Recombinant Mouse CD274/PD-L1/B7-H1 Protein (Cat No.:
MV974011) in indirect ELISA.
SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Mouse CD274/PD-L1/B7-H1 Antibody (10F.9G2).
Flow CytoMetry
Flow-cytometry using FITC anti-Mouse PD1 antibody. Unstimulated cells (blue Histogram) and 3-day CoA-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse PD1 monoclonal antibody (Catalog: MV974020) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.