Anti-Rat VEGFA Antibody (B20-4)
Overview
Catalog No.
RB941013
Host species
Mouse
Species reactivity
Human, Mouse, Rat, Rabbit
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
Blocking, ELISA
Target
Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P16612
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
B20-4
Note
For research use only.
Images
Bioactivity
Detects Rat VEGFA in indirect ELISAs.
SDS-PAGE
SDS-PAGE for Anti-Rat VEGFA Antibody (B20-4).
Flow CytoMetry
Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.