Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, Ig epsilon chain C region ND, IGHE
Purification
Protein A/G purified from cell culture supernatant.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SAA0343
Product Usage Information
Application
Dilution
ELISA
1:2000-1:20000
Note
For research use only.
Description
Anti-Human IgE/IGHE Antibody (SAA0343) [SAA0343] (HV064033) is a mouse monoclonal antibody detecting IGHE in ELISA, FCM. Suitable for Human.
Highlights
●Flow Cytometry — Validated for flow cytometry applications.
Images
Flow Cytometry
Flow-cytometry using anti-human IgE antibody.IgE Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human IgE antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human IgE/IGHE Antibody (SAA0343).