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Anti-Human ROBO1 Antibody (B5209B)
Overview
Catalog No. HP485023
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications ELISA
Target ROBO1, Roundabout homolog 1, H-Robo-1
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession Q9Y6N7
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDB5209B
Note For research use only.
Images


Flow CytoMetry
Flow-cytometry using FITC anti-human ROBO1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ROBO1 monoclonal antibody (Catalog: HP485023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Western blot
Western blot analysis was performed using anti-ROBO1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human ROBO1 (Catalog No: HP485011)
Lane 2: negative control