Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P25942
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
SEC-HPLC detection for Anti-Human CD40/TNFRSF5 Antibody (ABS2001).
Flow Cytometry
Flow-cytometry using anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD40 monoclonal antibody (Catalog HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human CD40/TNFRSF5 Antibody (SAA2006).
Flow CytoMetry
Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using PE anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.