| Catalog No. | HY328043 | ||||||
| Host species | Human | ||||||
| Species reactivity | Human | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS, pH 7.4. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | IgG4, kappa | ||||||
| Applications | ELISA, FCM, WB | ||||||
| Target | Hybridoma growth factor, BSF-2, IFN-beta-2, CDF, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, Interferon beta-2, IFNB2, CTL differentiation factor | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | P05231 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Clone ID | SAA2009 | ||||||
| Product Usage Information |
|
||||||
| Note | For research use only. | ||||||
| RRID | Anti-Human IL6 Antibody (SAA2009) (abinScience Cat# HY328043, RRID:AB_3727921) |
Anti-Human IL6 Antibody (SAA2009)
Overview
Description
Anti-Human IL6 Antibody (SAA2009) [SAA2009] (HY328043) is a human monoclonal antibody detecting IL6 in ELISA, FCM, WB. Suitable for Human.
Highlights
- ●Multi-Application — Validated across multiple applications.
- ●Flow Cytometry — Validated for flow cytometry applications.
Images
 Bioactivity |
SEC-HPLC detection for Anti-Human IL6 Antibody (ABS1221). |
 Flow Cytometry |
Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328043 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human IL6 Antibody (SAA2009). |
 Western blot |
Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng |