| Catalog No. | HY436013 | ||||
| Host species | Mouse | ||||
| Species reactivity | Human | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS, pH 7.4. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Clonality | Monoclonal | ||||
| Isotype | IgG2a, kappa | ||||
| Applications | WB | ||||
| Target | APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Accession | P07858 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Clone ID | 2A2 | ||||
| Product Usage Information |
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| Note | For research use only. | ||||
| RRID | Anti-Human CTSB Antibody (2A2) (abinScience Cat# HY436013, RRID:AB_3728963) |
Anti-Human CTSB Antibody (2A2)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Anti-Human CTSB Antibody (2A2). |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human CTSB (Catalog No: HY436012), 50ng Lane 2: recombinant human CTSB (Catalog No: HY436012), 30ng Lane 3: recombinant human CTSB (Catalog No: HY436012), 20ng |