Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B)
Overview
Catalog No.
JN072013
Host species
Mouse
Species reactivity
Escherichia coli O157:H7
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a
Applications
ELISA, FCM, WB
Target
stx1, stx1B, Shiga toxin 1 B subunit, Shiga toxin I B subunit, Escherichia coli O157:H7
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
Q7BQ98, Q32GM0, P69178, P69179, Q779K3
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
SEC-HPLC detection for Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B).
Western blot
Western blot analysis was performed using anti-stx1B monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant E. coli stx1B (Catalog No: JN072012) Lane 2: negative control
Bioactivity
Detects recombinant E. coli shiga toxin type 1 B/stx1B (Catalog No: JN072012) in indirect ELISA