Anti-Human Gal1/LGALS1 Antibody (8A12)
Overview
Catalog No.
HY272023
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
ELISA, FCM
Target
HLBP14, Lactose-binding lectin 1, LGALS1, HBL, HPL, Beta-galactoside-binding lectin L-14-I, Gal-1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, Galaptin, Galectin-1, 14 kDa laminin-binding protein, 14 kDa lectin
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P09382
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
8A12
Note
For research use only.
Images
Bioactivity
Detects Human Gal1/LGALS1 in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human Galectin-1 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human Galectin-1 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human Gal1/LGALS1 Antibody (8A12).