Rat Anti-Human GPC3 Antibody (YP7)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Applications
ELISA, FCM, WB
Target
GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Images
Bioactivity
Detects recombinant human Glypican-3/GPC3 (Cat No.:
HW310011) in indirect ELISA.
Bioactivity
Detects recombinant mouse Glypican-3/GPC3 (Cat No.:
MW310011) in indirect ELISA.
SDS-PAGE
SDS-PAGE for Rat Anti-Human GPC3 Antibody (YP7).
Flow CytoMetry
Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310053) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (abinScience: ?) and cells analysed on a NovoCyte Flow Cytometer.