InVivoMAb Anti-Human TSG101 Antibody (Iv0253)

Catalog No.	HV181010
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Neutralization
Target	Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	Q99816
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	Iv0253
Note	For research use only. Not suitable for clinical or therapeutic use.
RRID	InVivoMAb Anti-Human TSG101 Antibody (Iv0253) (abinScience Cat# HV181010, RRID:AB_3729553)

Western blot	Western blot analysis was performed using anti-TSG101 monoclonal antibody at 1ug/mL on various samples. Lane 1: SH-SY5Y cell lysate Lane 2: K562 cell lysate Lane 3: NIH3T3 cell lysate Lane 4: PC-12 cell lysate Lane 5: Lncap cell lysate Lane 6: 293T cell lysate Lane 7: Mouse brain lysate Lane 8: Rat brain lysate Lane 9: Rat heart lysate
Flow cytometry	Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.