Anti-Human DNMT3A IP Antibody (SAA0199)

Catalog No.	HV233013
Host species	Mouse
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG2a
Applications	ELISA, FCM, IF, IP
Target	Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	Q9Y6K1
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	SAA0199
Note	For research use only.

Flow Cytometry	Flow-cytometry using anti-human DNMT3A antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human DNMT3A antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human DNMT3A antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human DNMT3A antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human DNMT3A antibody monoclonal antibody (Catalog # HV233013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Anti-Human DNMT3A IP Antibody (SAA0199).
Flow CytoMetry	Flow-cytometry using FITC anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human DNMT3A antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human DNMT3A monoclonal antibody (Catalog: HV233013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.