Research Grade Anti-Human CD318/CDCP1 (RG7287)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% purity as determined by SDS-PAGE.
Applications
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target
CD318, Transmembrane and associated with src kinases, TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1, CDCP1
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact the lab for this information.
Expression system
Mammalian Cells
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
Bioactivity
Detects CD318 in indirect ELISA.
SDS-PAGE
SDS-PAGE for Research Grade Anti-Human CD318/CDCP1 (RG7287).
Flow CytoMetry
Flow-cytometry using APC anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using PE anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.