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Recombinant Human CD33 Protein, C-His
Overview
Catalog No. HB185011
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Purity >90% as determined by SDS-PAGE.
Applications ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level Please contact with the lab for this information.
Expression system Mammalian Cells
Accession P20138
Protein length Met1-His259
Nature Recombinant
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Alternative name Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3
Species Homo sapiens (Human)
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Predicted molecular weight 29.61 kDa
Note For research use only.
Description

Recombinant Human CD33 Protein, C-His (HB185011) expressed in Mammalian Cells, spanning Met1-His259. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.

Images


SDS-PAGE		 SDS-PAGE for Recombinant Human CD33 Protein, C-His

Bioactivity		 Detects Lintuzumab in indirect ELISAs.

Bioactivity		 Detected by Research Grade Vadastuximab (Cat No.: HB185036) in indirect ELISA.

Bioactivity		 Detected by Research Grade Anti-Human CD33 (BI 836858) (Catalog No: HB185076) in indirect ELISA

Bioactivity		 Detected by SAA2052 (Catalog No: HB185013) in indirect ELISA.

Bioactivity		 Detected by Avitotamig (Catalog No: HB185096) in indirect ELISA.