Recombinant Human CD279/PDCD1/PD1 Protein, C-His

Catalog No.	HS870021
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>90% as determined by SDS-PAGE.
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level	Please contact with the lab for this information.
Expression system	Mammalian Cells
Accession	Q15116
Protein length	Pro21-Gln167
Nature	Recombinant
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Alternative name	Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1, CD279
Species	Homo sapiens (Human)
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Predicted molecular weight	19.73 kDa
Note	For research use only.

Recombinant Human CD279/PDCD1/PD1 Protein, C-His (HS870021) expressed in Mammalian Cells, spanning Pro21-Gln167. Purity: >90% by SDS-PAGE.

Highlights

●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.

SDS-PAGE	SDS-PAGE for Recombinant Human CD279/PDCD1/PD1 Protein, C-His
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Bioactivity	Detects Vudalimab in indirect ELISAs.
Bioactivity	Detects Rosnilimab in indirect ELISAs.
Bioactivity	Detects Nofazinlimab in indirect ELISAs.
Bioactivity	Detects Camrelizumab in indirect ELISAs.
Bioactivity	Detected by Research Grade Reozalimab in indirect ELISA.
Bioactivity	Detected by Research Grade Volrustomig (Cat No.: HS870396) inindirectELISA.
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